Synthesis and molecular recognition of phosphatidylinositol-3-methylenephosphate.

ABSTRACT: [reaction: see text] Phosphatidylinositol-3-phosphate (PtdIns(3)P) is a spatial regulator of vesicular trafficking and other vital cellular processes. We describe the asymmetric total synthesis of a metabolically stabilized analogue, phosphatidylinositol-3-methylenephosphate (PtdIns(3)MP) from a differentially protected myo-inositol. NMR studies of PtdIns(3)MP bound to the (15)N-labeled FYVE domain showed significant (1)H and (15)N chemical shift changes relative to the unliganded protein.

SUBMITTER: Gajewiak J 

PROVIDER: S-EPMC2535797 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature