Effects of modified digestion schemes on the identification of proteins from complex mixtures.

ABSTRACT: In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.

SUBMITTER: Klammer AA 

PROVIDER: S-EPMC2535816 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature