A novel transposon, Tn6009, composed of a Tn916 element linked with a Staphylococcus aureus mer operon.

ABSTRACT:

Objectives

The aim of this study was to characterize a novel conjugative transposon Tn6009 composed of a Tn916 linked to a Staphylococcus aureus mer operon in representative Gram-positive and Gram-negative bacteria isolated in Nigeria and Portugal.

Methods

Eighty-three Gram-positive and 34 Gram-negative bacteria were screened for the presence of the Tn6009 using DNA-DNA hybridization, PCR, hybridization of PCR products, sequencing and mating experiments by established procedures.

Results

Forty-three oral and 23 urine Gram-negative and Gram-positive isolates carried the Tn6009. Sequencing was performed to verify the direct linkage between the mer resistance genes and the tet(M) gene. A Nigerian Klebsiella pneumoniae, isolated from a urinary tract infection patient, and

SUBMITTER: Soge OO 

PROVIDER: S-EPMC2536709 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature