Constraints imposed by non-functional protein-protein interactions on gene expression and proteome size.

ABSTRACT: Crowded intracellular environments present a challenge for proteins to form functional specific complexes while reducing non-functional interactions with promiscuous non-functional partners. Here we show how the need to minimize the waste of resources to non-functional interactions limits the proteome diversity and the average concentration of co-expressed and co-localized proteins. Using the results of high-throughput yeast 2-hybrid experiments, we estimate the characteristic strength of non-functional protein-protein interactions. By combining these data with the strengths of specific interactions, we assess the fraction of time proteins spend tied up in non-functional interactions as a function of their overall concentration. This allows us to sketch the phase diagram for baker's yeast

SUBMITTER: Zhang J 

PROVIDER: S-EPMC2538908 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature