Activation of utrophin promoter by heregulin via the ets-related transcription factor complex GA-binding protein alpha/beta.

ABSTRACT: Utrophin/dystrophin-related protein is the autosomal homologue of the chromosome X-encoded dystrophin protein. In adult skeletal muscle, utrophin is highly enriched at the neuromuscular junction. However, the molecular mechanisms underlying regulation of utrophin gene expression are yet to be defined. Here we demonstrate that the growth factor heregulin increases de novo utrophin transcription in muscle cell cultures. Using mutant reporter constructs of the utrophin promoter, we define the N-box region of the promoter as critical for heregulin-mediated activation. Using this region of the utrophin promoter for DNA affinity purification, immunoblots, in vitro kinase assays, electrophoretic mobility shift assays, and in vitro expression in cultured muscle cells, we demonstrate that ets-relat

SUBMITTER: Khurana TS 

PROVIDER: S-EPMC25417 | biostudies-literature | 1999 Jun

REPOSITORIES: biostudies-literature