Airway lipoxin A4 generation and lipoxin A4 receptor expression are decreased in severe asthma.

ABSTRACT:

Rationale

Airway inflammation is common in severe asthma despite antiinflammatory therapy with corticosteroids. Lipoxin A(4) (LXA(4)) is an arachidonic acid-derived mediator that serves as an agonist for resolution of inflammation.

Objectives

Airway levels of LXA(4), as well as the expression of lipoxin biosynthetic genes and receptors, in severe asthma.

Methods

Samples of bronchoalveolar lavage fluid were obtained from subjects with asthma and levels of LXA(4) and related eicosanoids were measured. Expression of lipoxin biosynthetic genes was determined in whole blood, bronchoalveolar lavage cells, and endobronchial biopsies by quantitative polymerase chain reaction, and leukocyte LXA(4) receptors were monitored by flow cytometry.

Measurements and main results

SUBMITTER: Planaguma A 

PROVIDER: S-EPMC2542432 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature