Spatial mapping of the neurite and soma proteomes reveals a functional Cdc42/Rac regulatory network.

ABSTRACT: Neurite extension and growth cone navigation are guided by extracellular cues that control cytoskeletal rearrangements. However, understanding the complex signaling mechanisms that mediate neuritogenesis has been limited by the inability to biochemically separate the neurite and soma for spatial proteomic and bioinformatic analyses. Here, we apply global proteome profiling in combination with a neurite purification methodology for comparative analysis of the soma and neurite proteomes of neuroblastoma cells. The spatial relationship of 4,855 proteins were mapped, revealing networks of signaling proteins that control integrins, the actin cytoskeleton, and axonal guidance in the extending neurite. Bioinformatics and functional analyses revealed a spatially compartmentalized Rac/Cdc42 signali

SUBMITTER: Pertz OC 

PROVIDER: S-EPMC2542865 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature