Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization.

ABSTRACT: Microarrays of virus-specific oligonucleotides may provide a method of screening samples for the presence or absence of a large variety of viruses simultaneously. Influenza viruses are ideal for evaluating such microarrays because of their genetic and host diversity, and the availability of an extensive sequence database. A collection of 476 influenza virus-specific oligonucleotides was spotted onto glass slides as probes. Viral RNAs were reverse transcribed and amplified by PCR, and the products were labeled with cyanine dyes. The presence of viruses and their identities were determined by hybridization. The fluorescence intensities of oligonucleotide spots were highly reproducible within each slide and satisfactorily proportional between experiments. However, the intensities of probe spo

SUBMITTER: Sengupta S 

PROVIDER: S-EPMC254299 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature