Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform.

ABSTRACT: Tagging of proteins in vivo by covalent attachment of a biotin moiety has emerged as a new prospective tool for protein detection and purification. Previously, we established a strategy for expression of in vivo biotinylated proteins in mammalian cells. It is based on coexpression of the protein of interest fused to a short biotin acceptor peptide and biotin ligase BirA cloned in the same vector. We show here that the in vivo biotinylation can be used for immunogold postembedding labeling in immunoelectron microscopy experiments. We show that immunoelectron microscopy with biotinylated nuclear proteins is compatible with a wide range of postembedding methods, facilitating combination of morphological and localization studies in a single experiment. We also show that the method works in bot

SUBMITTER: Viens A 

PROVIDER: S-EPMC2544619 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature