Comparative study of sugar fermentation and protein expression patterns of two Lactobacillus plantarum strains grown in three different media.

ABSTRACT: A comparative study of two strains of Lactobacillus plantarum (REB1 and MLBPL1) grown in commercial medium (MRS broth), cucumber juice, and liquid pig feed was performed to explore changes to the metabolic pathways of these bacteria, using a proteomics approach (two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry) combined with analyses of fermentable sugars and fermentation end products. The protein expression showed that even with an excess of glucose in all media, both strains could metabolize different carbohydrates simultaneously and that hexoses could also be used via a phosphoketolase pathway with preferential expression in liquid feed. Sugar analyses showed that the fermentation of sugars was homolactic for all media, with some heterolactic activity i

SUBMITTER: Plumed-Ferrer C 

PROVIDER: S-EPMC2546631 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature