Dissecting colony development of Neurospora crassa using mRNA profiling and comparative genomics approaches.

ABSTRACT: Colony development, which includes hyphal extension, branching, anastomosis, and asexual sporulation, is a fundamental aspect of the life cycle of filamentous fungi; genetic mechanisms underlying these phenomena are poorly understood. We conducted transcriptional profiling during colony development of the model filamentous fungus Neurospora crassa, using 70-mer oligonucleotide microarrays. Relative mRNA expression levels were determined for six sections of defined age excised from a 27-h-old N. crassa colony. Functional category analysis showed that the expression of genes involved in cell membrane biosynthesis, polar growth, and cellular signaling was enriched at the periphery of the colony. The relative expression of genes involved in protein synthesis and energy production was enriched

SUBMITTER: Kasuga T 

PROVIDER: S-EPMC2547062 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature