Measuring microRNAs: comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods.

ABSTRACT:

Background

Determining the expression levels of microRNAs (miRNAs) is of great interest to researchers in many areas of biology, given the significant roles these molecules play in cellular regulation. Two common methods for measuring miRNAs in a total RNA sample are microarrays and quantitative RT-PCR (qPCR). To understand the results of studies that use these two different techniques to measure miRNAs, it is important to understand how well the results of these two analysis methods correlate. Since both methods use total RNA as a starting material, it is also critical to understand how measurement of miRNAs might be affected by the particular method of total RNA preparation used.

Results

We measured the expression of 470 human miRNAs in nine human tissues using Agilent mic

SUBMITTER: Ach RA 

PROVIDER: S-EPMC2547107 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature