Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii.

ABSTRACT:

Background

Phosphoribosyl pyrophosphate (PRPP) is a central compound for cellular metabolism and may be considered as a link between carbon and nitrogen metabolism. PRPP is directly involved in the de novo and salvage biosynthesis of GTP, which is the immediate precursor of riboflavin. The industrial production of this vitamin using the fungus Ashbya gossypii is an important biotechnological process that is strongly influenced by substrate availability.

Results

Here we describe the characterization and manipulation of two genes of A. gossypii encoding PRPP synthetase (AGR371C and AGL080C). We show that the AGR371C and AGL080C gene products participate in PRPP synthesis and exhibit inhibition by ADP. We also observed a major contribution of AGL080C to total PRPP synthetase ac

SUBMITTER: Jimenez A 

PROVIDER: S-EPMC2551608 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature