Gene organization, evolution and expression of the microtubule-associated protein ASAP (MAP9).

ABSTRACT:

Background

ASAP is a newly characterized microtubule-associated protein (MAP) essential for proper cell-cycling. We have previously shown that expression deregulation of human ASAP results in profound defects in mitotic spindle formation and mitotic progression leading to aneuploidy, cytokinesis defects and/or cell death. In the present work we analyze the structure and evolution of the ASAP gene, as well as the domain composition of the encoded protein. Mouse and Xenopus cDNAs were cloned, the tissue expression characterized and the overexpression profile analyzed.

Results

Bona fide ASAP orthologs are found in vertebrates with more distantly related potential orthologs in invertebrates. This single-copy gene is conserved in mammals where it maps to syntenic chromosomal regi

SUBMITTER: Venoux M 

PROVIDER: S-EPMC2551623 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature