Mapping local matrix remodeling induced by a migrating tumor cell using three-dimensional multiple-particle tracking.

ABSTRACT: Mesenchymal cell migration through a three-dimensional (3D) matrix typically involves major matrix remodeling. The direction of matrix deformation occurs locally in all three dimensions, which cannot be measured by current techniques. To probe the local, 3D, real-time deformation of a collagen matrix during tumor cell migration, we developed an assay whereby matrix-embedded beads are tracked simultaneously in all three directions with high resolution. To establish a proof of principle, we investigated patterns of collagen I matrix deformation near fibrosarcoma cells in the absence and presence of inhibitors of matrix metalloproteinases and acto-myosin contractility. Our results indicate that migrating cells show patterns of local matrix deformation toward the cell that are symmetric in mag

SUBMITTER: Bloom RJ 

PROVIDER: S-EPMC2553112 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature