Transgenic, fluorescent Leishmania mexicana allow direct analysis of the proteome of intracellular amastigotes.

ABSTRACT: Investigating the proteome of intracellular pathogens is often hampered by inadequate methodologies to purify the pathogen free of host cell material. This has also precluded direct proteome analysis of the intracellular, amastigote form of Leishmania spp., protozoan parasites that cause a spectrum of diseases that affect some 12 million patients worldwide. Here a method is presented that combines classic, isopycnic density centrifugation with fluorescent particle sorting for purification by exploiting transgenic, fluorescent parasites to allow direct proteome analysis of the purified organisms. By this approach the proteome of intracellular Leishmania mexicana amastigotes was compared with that of extracellular promastigotes that are transmitted by insect vectors. In total, 509 different

SUBMITTER: Paape D 

PROVIDER: S-EPMC2556020 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature