Robust and sensitive iTRAQ quantification on an LTQ Orbitrap mass spectrometer.

ABSTRACT: Isobaric stable isotope tagging reagents such as tandem mass tags or isobaric tags for relative and absolute quantification enable multiplexed quantification of peptides via reporter ion signals in the low mass range of tandem mass spectra. Until recently, the poor recovery of low mass fragments observed in tandem mass spectra acquired on ion trap mass spectrometers precluded the use of these reagents on this widely available instrument platform. The Pulsed Q Dissociation (PQD) technique allows negotiating this limitation but suffers from poor fragmentation efficiency, which has raised doubts in the community as to its practical utility. Here we show that by carefully optimizing instrument parameters such as collision energy, activation Q, delay time, ion isolation width, number of microsc

SUBMITTER: Bantscheff M 

PROVIDER: S-EPMC2556025 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature