High throughput selection of effective serodiagnostics for Trypanosoma cruzi infection.

ABSTRACT:

Background

Diagnosis of Trypanosoma cruzi infection by direct pathogen detection is complicated by the low parasite burden in subjects persistently infected with this agent of human Chagas disease. Determination of infection status by serological analysis has also been faulty, largely due to the lack of well-characterized parasite reagents for the detection of anti-parasite antibodies.

Methods

In this study, we screened more than 400 recombinant proteins of T. cruzi, including randomly selected and those known to be highly expressed in the parasite stages present in mammalian hosts, for the ability to detect anti-parasite antibodies in the sera of subjects with confirmed or suspected T. cruzi infection.

Findings

A set of 16 protein groups were identified and incorpora

SUBMITTER: Cooley G 

PROVIDER: S-EPMC2556098 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature