Identification of direct regulatory targets of the transcription factor Sox10 based on function and conservation.

ABSTRACT:

Background

Sox10, a member of the Sry-related HMG-Box gene family, is a critical transcription factor for several important cell lineages, most notably the neural crest stem cells and the derivative peripheral glial cells and melanocytes. Thus far, only a handful of direct target genes are known for this transcription factor limiting our understanding of the biological network it governs.

Results

We describe identification of multiple direct regulatory target genes of Sox10 through a procedure based on function and conservation. By combining RNA interference technique and DNA microarray technology, we have identified a set of genes that show significant down-regulation upon introduction of Sox10 specific siRNA into Schwannoma cells. Subsequent comparative genomics analyses l

SUBMITTER: Lee KE 

PROVIDER: S-EPMC2556353 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature