Role of IFN-gamma and IL-6 in a protective immune response to Yersinia enterocolitica in mice.

ABSTRACT:

Background

Yersinia outer protein (Yop) H is a secreted virulence factor of Yersinia enterocolitica (Ye), which inhibits phagocytosis of Ye and contributes to the virulence of Ye in mice. The aim of this study was to address whether and how YopH affects the innate immune response to Ye in mice.

Results

For this purpose, mice were infected with wild type Ye (pYV+) or a YopH-deficient Ye mutant strain (DeltayopH). CD11b+ cells were isolated from the infected spleen and subjected to gene expression analysis using microarrays. Despite the attenuation of DeltayopH in vivo, by variation of infection doses we were able to achieve conditions that allow comparison of gene expression in pYV+ and DeltayopH infection, using either comparable infection courses or splenic bacterial burden

SUBMITTER: Matteoli G 

PROVIDER: S-EPMC2556677 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature