N-Glycan structure annotation of glycopeptides using a linearized glycan structure database (GlyDB).

ABSTRACT: While glycoproteins are abundant in nature, and changes in glycosylation occur in cancer and other diseases, glycoprotein characterization remains a challenge due to the structural complexity of the biopolymers. This paper presents a general strategy, termed GlyDB, for glycan structure annotation of N-linked glycopeptides from tandem mass spectra in the LC-MS analysis of proteolytic digests of glycoproteins. The GlyDB approach takes advantage of low-energy collision-induced dissociation of N-linked glycopeptides that preferentially cleaves the glycosidic bonds while the peptide backbone remains intact. A theoretical glycan structure database derived from biosynthetic rules for N-linked glycans was constructed employing a novel representation of branched glycan structures consisting of mult

SUBMITTER: Ren JM 

PROVIDER: S-EPMC2557434 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature