Detection and genotyping of Giardia intestinalis isolates using intergenic spacers(IGS)-based PCR.

ABSTRACT: Giardia intestinalis infections arise primarily from contaminated food or water. Zoonotic transmission is possible, and at least 7 major assemblages including 2 assemblages recovered from humans have been identified. The determination of the genotype of G. intestinalis is useful not only for assessing the correlation of clinical symptoms and genotypes, but also for finding the infection route and its causative agent in epidemiological studies. In this study, methods to identify the genotypes more specifically than the known 2 genotypes recovered from humans have been developed using the intergenic spacer (IGS) region of rDNA. The IGS region contains varying sequences and is thus suitable for comparing isolates once they are classified as the same strain. Genomic DNA was extracted from cyst

SUBMITTER: Lee JH 

PROVIDER: S-EPMC2559131 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature