A novel reverse transduction adenoviral array for the functional analysis of shRNA libraries.

ABSTRACT:

Background

The identification of novel drug targets by assessing gene functions is most conveniently achieved by high-throughput loss-of-function RNA interference screening. There is a growing need to employ primary cells in such screenings, since they reflect the physiological situation more closely than transformed cell lines do. Highly miniaturized and parallelized approaches as exemplified by reverse transfection or transduction arrays meet these requirements, hence we verified the applicability of an adenoviral microarray for the elucidation of gene functions in primary cells.

Results

Here, we present microarrays of infectious adenoviruses encoding short hairpin RNA (shRNA) as a new tool for gene function analysis. As an example to demonstrate its application, we chose

SUBMITTER: Oehmig A 

PROVIDER: S-EPMC2559852 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature