Evaluation of suitable reference genes for gene expression studies in bovine muscular tissue.

ABSTRACT:

Background

Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RTqPCR) is a technique used to measure mRNA species copy number as a way to determine key genes involved in different biological processes. However, the expression level of these key genes may vary among tissues or cells not only as a consequence of differential expression but also due to different factors, including choice of reference genes to normalize the expression levels of the target genes; thus the selection of reference genes is critical for expression studies. For this purpose, ten candidate reference genes were investigated in bovine muscular tissue.

Results

The value of stability of ten candidate reference genes included in three groups was estimated: the so called 'class

SUBMITTER: Perez R 

PROVIDER: S-EPMC2561043 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature