ITS-2 secondary structures and phylogeny of Anopheles culicifacies species.

ABSTRACT:

Background

Second internal transcribed spacer (ITS2) has proven to contain useful biological information at higher taxonomic levels.

Objectives

This study was carried out to unravel the biological information in the ITS2 region of An. culicifacies and the internal relationships between the five species of Anopheles culicifacies.

Methodology

In achieving these objectives, twenty two ITS2 sequences (approximately 370bp) of An. culicifacies species were retrieved from GenBank and secondary structures were generated. For the refinement of the primary structures, i.e. nucleotide sequence of ITS2 sequences, generated secondary structures were used. The improved ITS2 primary structures sequences were then aligned and used for the construction of phylogenetic trees.

Result

SUBMITTER: Dassanayake RS 

PROVIDER: S-EPMC2561166 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature