Rho kinase-dependent CCL20 induced by dynamic compression of human chondrocytes.

ABSTRACT:

Objective

Mechanical stimulation of cartilage affects tissue homeostasis and chondrocyte function. The chondrocyte phenotype is dependent on cell shape, which is largely determined by the actin cytoskeleton. Reorganization of the actin cytoskeleton results from Rho GTPase activation. The purpose of this study was to examine the roles of both actin and Rho in mechanotransduction in chondrocytes.

Methods

We embedded human articular chondrocytes in 2 x 6-mm agarose discs at 5 x 10(6) cells/ml and subjected the discs to unconfined dynamic compression at 0.5 Hz. By comparing samples with and without dynamic compression, we identified Rho activation according to the GTP-bound active RhoA measured in cell lysates. We identified rearrangements in filamentous actin structures using f

SUBMITTER: Haudenschild DR 

PROVIDER: S-EPMC2562294 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature