Integration of 18O labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins.

ABSTRACT: Forward and reverse (18)O labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, 12 were detected with abundances altered at least 2-fold.

SUBMITTER: Wang J 

PROVIDER: S-EPMC2562304 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature