Large-scale identification of polymorphic microsatellites using an in silico approach.

ABSTRACT:

Background

Simple Sequence Repeat (SSR) or microsatellite markers are valuable for genetic research. Experimental methods to develop SSR markers are laborious, time consuming and expensive. In silico approaches have become a practicable and relatively inexpensive alternative during the last decade, although testing putative SSR markers still is time consuming and expensive. In many species only a relatively small percentage of SSR markers turn out to be polymorphic. This is particularly true for markers derived from expressed sequence tags (ESTs). In EST databases a large redundancy of sequences is present, which may contain information on length-polymorphisms in the SSR they contain, and whether they have been derived from heterozygotes or from different genotypes. Up to now, alth

SUBMITTER: Tang J 

PROVIDER: S-EPMC2562394 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature