Gene profiling and signaling pathways of Candida albicans keratitis.

ABSTRACT:

Purpose

To compare the global gene expression patterns in uninfected and fungus-infected mouse corneas at the onset of Candida albicans keratitis.

Methods

Fungal keratitis was generated by scarifying the corneal epithelium of BALB/c mice followed by topical inoculation with Candida albicans. Corneal infection was allowed to progress for one day, and total RNA was then extracted from excised corneas. Microarray was performed to detect 45,102 murine genes and processed to identify genetic regulation of signaling pathways. Selected genes encoding interleukins (IL), chemokine ligands, and other cytokines were confirmed by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).

Results

Compared to mock-inoculated control eyes, genetic microarray an

SUBMITTER: Yuan X 

PROVIDER: S-EPMC2562425 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature