Phylogenetic analysis of Clostridium botulinum type A by multi-locus sequence typing.

ABSTRACT: The genus Clostridium comprises a heterogeneous group of organisms for which the phylogeny and evolutionary relationships are poorly understood. The elucidation of these evolutionary relationships necessitates the use of experimental methods that can distinguish Clostridium lineages that are time and cost effective, and can be accurately and reproducibly employed in different laboratories. Multi-locus sequence typing (MLST) has been successfully used as a reproducible and discriminating system in the study of eukaryotic and prokaryotic evolutionary biology, and for strain typing of various bacteria. In this study, MLST was applied to evaluate the evolutionary lineages in the serotype A group of Clostridium botulinum. C. botulinum type A has recently been shown to produce multiple subtypes,

SUBMITTER: Jacobson MJ 

PROVIDER: S-EPMC2562668 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature