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BLyS inhibition eliminates primary B cells but leaves natural and acquired humoral immunity intact.

ABSTRACT: We have used an inhibiting antibody to determine whether preimmune versus antigen-experienced B cells differ in their requisites for BLyS, a cytokine that controls differentiation and survival. Whereas in vivo BLyS inhibition profoundly reduced naïve B cell numbers and primary immune responses, it had a markedly smaller effect on memory B cells and long-lived plasma cells, as well as secondary immune responses. There was heterogeneity within the memory pools, because IgM-bearing memory cells were sensitive to BLyS depletion whereas IgG-bearing memory cells were not, although both were more resistant than naïve cells. There was also heterogeneity within B1 pools, as splenic but not peritoneal B1 cells were diminished by anti-BLyS treatment, yet the number of natural antibody-secreting cells remained constant. Together, these findings show that memory B cells and natural antibody-secreting cells are BLyS-independent and suggest that these pools can be separately manipulated.

SUBMITTER: Scholz JL

PROVIDER: S-EPMC2563088 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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BLyS inhibition eliminates primary B cells but leaves natural and acquired humoral immunity intact.

Scholz Jean L JL Crowley Jenni E JE Tomayko Mary M MM Steinel Natalie N O'Neill Patrick J PJ Quinn William J WJ Goenka Radhika R Miller Juli P JP Cho Yun Hee YH Long Vatana V Ward Chris C Migone Thi-Sau TS Shlomchik Mark J MJ Cancro Michael P MP

Proceedings of the National Academy of Sciences of the United States of America 20081001 40

We have used an inhibiting antibody to determine whether preimmune versus antigen-experienced B cells differ in their requisites for BLyS, a cytokine that controls differentiation and survival. Whereas in vivo BLyS inhibition profoundly reduced naïve B cell numbers and primary immune responses, it had a markedly smaller effect on memory B cells and long-lived plasma cells, as well as secondary immune responses. There was heterogeneity within the memory pools, because IgM-bearing memory cells wer ...[more]

PMID: 18832171

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Dataset Information

BLyS inhibition eliminates primary B cells but leaves natural and acquired humoral immunity intact.

Publications

BLyS inhibition eliminates primary B cells but leaves natural and acquired humoral immunity intact.

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