Small RNA regulation of ovule development in the cotton plant, G. hirsutum L.

ABSTRACT:

Background

The involvement of small RNAs in cotton fiber development is under explored. The objective of this work was to directly clone, annotate, and analyze small RNAs of developing ovules to reveal the candidate small interfering RNA/microRNAs involved in cotton ovule and fiber development.

Results

We cloned small RNA sequences from 0-10 days post anthesis (DPA) developing cotton ovules. A total of 6691 individual colonies were sequenced from 11 ovule small RNA libraries that yielded 2482 candidate small RNAs with a total of 583 unique sequence signatures. The majority (362, 62.1%) of these 583 sequences were 24 nt long with an additional 145 sequences (24.9%) in the 21 nt to 23 nt size range. Among all small RNA sequence signatures only three mirBase-confirmed plant mic

SUBMITTER: Abdurakhmonov IY 

PROVIDER: S-EPMC2564936 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature