Use of genomic DNA as an indirect reference for identifying gender-associated transcripts in morphologically identical, but chromosomally distinct, Schistosoma mansoni cercariae.

ABSTRACT:

Background

The use of DNA microarray technology to study global Schistosoma gene expression has led to the rapid identification of novel biological processes, pathways or associations. Implementation of standardized DNA microarray protocols across laboratories would assist maximal interpretation of generated datasets and extend productive application of this technology.

Methodology/principal findings

Utilizing a new Schistosoma mansoni oligonucleotide DNA microarray composed of 37,632 elements, we show that schistosome genomic DNA (gDNA) hybridizes with less variation compared to complex mixed pools of S. mansoni cDNA material (R = 0.993 for gDNA compared to R = 0.956 for cDNA during 'self versus self' hybridizations). Furthermore, these effects are species-specific, with S.

SUBMITTER: Fitzpatrick JM 

PROVIDER: S-EPMC2565838 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature