Optimization of metabolic capacity and flux through environmental cues to maximize hydrogen production by the cyanobacterium "Arthrospira (Spirulina) maxima".

ABSTRACT: Environmental and nutritional conditions that optimize the yield of hydrogen (H(2)) from water using a two-step photosynthesis/fermentation (P/F) process are reported for the hypercarbonate-requiring cyanobacterium "Arthrospira maxima." Our observations lead to four main conclusions broadly applicable to fermentative H(2) production by bacteria: (i) anaerobic H(2) production in the dark from whole cells catalyzed by a bidirectional [NiFe] hydrogenase is demonstrated to occur in two temporal phases involving two distinct metabolic processes that are linked to prior light-dependent production of NADPH (photosynthetic) and dark/anaerobic production of NADH (fermentative), respectively; (ii) H(2) evolution from these reductants represents a major pathway for energy production (ATP) during ferm

SUBMITTER: Ananyev G 

PROVIDER: S-EPMC2565975 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature