Molecular detection of fungal communities in the Hawaiian marine sponges Suberites zeteki and Mycale armata.

ABSTRACT: Symbiotic microbes play a variety of fundamental roles in the health and habitat ranges of their hosts. While prokaryotes in marine sponges have been broadly characterized, the diversity of sponge-inhabiting fungi has barely been explored using molecular approaches. Fungi are an important component of many marine and terrestrial ecosystems, and they may be an ecologically significant group in sponge-microbe interactions. This study tested the feasibility of using existing fungal primers for molecular analysis of sponge-associated fungal communities. None of the eight selected primer pairs yielded satisfactory results in fungal rRNA gene or internal transcribed spacer (ITS) clone library constructions. However, 3 of 10 denaturing gradient gel electrophoresis (DGGE) primer sets, which were d

SUBMITTER: Gao Z 

PROVIDER: S-EPMC2565983 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature