Identification of transcripts involved in meiosis and follicle formation during ovine ovary development.

ABSTRACT:

Background

The key steps in germ cell survival during ovarian development are the entry into meiosis of oogonies and the formation of primordial follicles, which then determine the reproductive lifespan of the ovary. In sheep, these steps occur during fetal life, between 55 and 80 days of gestation, respectively. The aim of this study was to identify differentially expressed ovarian genes during prophase I meiosis and early folliculogenesis in sheep.

Results

In order to elucidate the molecular events associated with early ovarian differentiation, we generated two ovary stage-specific subtracted cDNA libraries using SSH. Large-scale sequencing of these SSH libraries identified 6,080 ESTs representing 2,535 contigs. Clustering and assembly of these ESTs resulted in a total of

SUBMITTER: Baillet A 

PROVIDER: S-EPMC2566313 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature