The long march: a sample preparation technique that enhances contig length and coverage by high-throughput short-read sequencing.

ABSTRACT: High-throughput short-read technologies have revolutionized DNA sequencing by drastically reducing the cost per base of sequencing information. Despite producing gigabases of sequence per run, these technologies still present obstacles in resequencing and de novo assembly applications due to biased or insufficient target sequence coverage. We present here a simple sample preparation method termed the "long march" that increases both contig lengths and target sequence coverage using high-throughput short-read technologies. By incorporating a Type IIS restriction enzyme recognition motif into the sequencing primer adapter, successive rounds of restriction enzyme cleavage and adapter ligation produce a set of nested sub-libraries from the initial amplicon library. Sequence reads from these su

SUBMITTER: Sorber K 

PROVIDER: S-EPMC2566813 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature