In silico identification of short nucleotide sequences associated with gene expression of pollen development in rice.

ABSTRACT: Microarray analysis of tiny amounts of RNA extracted from plant section samples prepared by laser microdissection (LM) can provide high-quality information on gene expression in specified plant cells at various stages of development. Having joined the LM-microarray analysis project, we utilized such genome-wide gene expression data from developing rice pollen cells to identify candidates for cis-regulatory elements for specific gene expression in these cells. We first found a few clusters of gene expression patterns based on the data from LM-microarrays. On one gene cluster in which the members were specifically expressed at the bicellular and mature pollen mitotic stages, we identified gene cluster fingerprints (GCFs), each of which consists of a short nucleotide representing the gene clu

SUBMITTER: Mihara M 

PROVIDER: S-EPMC2566928 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature