Identification of kaonashi mutants showing abnormal pollen exine structure in Arabidopsis thaliana.

ABSTRACT: Exine, the outermost architecture of pollen walls, protects male gametes from the environment by virtue of its chemical and physical stability. Although much effort has been devoted to revealing the mechanism of exine construction, still little is known about it. To identify the genes involved in exine formation, we screened for Arabidopsis mutants with pollen grains exhibiting abnormal exine structure using scanning electron microscopy. We isolated 12 mutants, kaonashi1 (kns1) to kns12, and classified them into four types. The type 1 mutants showed a collapsed exine structure resembling a mutant of the callose synthase gene, suggesting that the type 1 genes are involved in callose wall synthesis. The type 2 mutant showed remarkably thin exine structure, presumably due to defective primexi

SUBMITTER: Suzuki T 

PROVIDER: S-EPMC2566931 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature