Arteriolar genesis and angiogenesis induced by endothelial nitric oxide synthase overexpression results in a mature vasculature.

ABSTRACT:

Background

Generation of physiologically active vascular beds by delivery of combinations of growth factors offers promise for vascular gene therapy.

Methods and results

In a mesenteric model of physiological angiogenesis, combining endothelial nitric oxide synthase (eNOS) (and hence NO production) with VEGF and angiopoietin-1 overexpression resulted in a more functional vascular phenotype than growth factor administration alone. eNOS gene delivery upregulated eNOS, VEGF, and Ang-1 to similar levels as gene transfer with VEGF or Ang-1. eNOS overexpression resulted in neovascularization to a similar extent as VEGF and Ang-1 combined, but not by sprouting angiogenesis. Whereas combining Ang-1 and VEGF increased both exchange vessels and conduit vessels, neither growth factor n

SUBMITTER: Benest AV 

PROVIDER: S-EPMC2566940 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature