Susceptibility loci involved in cisplatin-induced cytotoxicity and apoptosis.

ABSTRACT:

Objectives

Cisplatin is a widely used chemotherapeutic agent; however, nephrotoxicity and neuropathy are obstacles for drug efficacy. Little is known about the genes or genetic variants contributing to the risk of developing these toxicities or chemotherapeutic response. Thus, we have applied a cell-based model to identify and characterize previously unknown genes that may be involved in cellular susceptibility to cisplatin.

Methods

Lymphoblastoid cell lines from 27 large Centre d'Etude du Polymorphisme Humain pedigrees were used to elucidate the genetic contribution to cisplatin-induced cytotoxicity. Phenotype was defined as cell growth inhibition following exposure of cell lines to increasing concentrations of cisplatin for 48 h.

Results

Significant heritability, ra

SUBMITTER: Shukla SJ 

PROVIDER: S-EPMC2567113 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature