Dynamical analysis on gene activity in the presence of repressors and an interfering promoter.

ABSTRACT: Transcription is regulated through interplay among transcription factors, an RNA polymerase (RNAP), and a promoter. Even for a simple repressive transcription factor that disturbs promoter activity at initial binding of RNAP, its repression level is not determined solely by the dissociation constant of transcription factor but is sensitive to timescales of processes in RNAP. We first analyze the promoter activity under strong repression by a slow binding repressor, in which case transcription events occur in bursts, followed by long quiescent periods while a repressor binds to the operator; the number of transcription events, bursting, and quiescent times are estimated by reaction rates. We then examine interference effect from an opposing promoter, using the correlation function of initia

SUBMITTER: Nakanishi H 

PROVIDER: S-EPMC2567934 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature