Structure, dynamics, and branch migration of a DNA Holliday junction: a single-molecule fluorescence and modeling study.

ABSTRACT: The Holliday junction (HJ) is a central intermediate of various genetic processes, including homologous and site-specific DNA recombination and DNA replication. Elucidating the structure and dynamics of HJs provides the basis for understanding the molecular mechanisms of these genetic processes. Our previous single-molecule fluorescence studies led to a model according to which branch migration is a stepwise process consisting of consecutive migration and folding steps. These data led us to the conclusion that one hop can be more than 1 basepair (bp); moreover, we hypothesized that continuous runs over the entire sequence homology (5 bp) can occur. Direct measurements of the dependence of the fluorescence resonance energy transfer (FRET) value on the donor-acceptor (D-A) distance are requi

SUBMITTER: Karymov MA 

PROVIDER: S-EPMC2567953 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature