Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?

ABSTRACT:

Background

The use of human embryonic stem cells (hESCs) in research is increasing and hESCs hold the promise for many biological, clinical and toxicological studies. Human ESCs are expected to be chromosomally stable since karyotypic changes represent a pitfall for potential future applications. Recently, several studies have analysed the genomic stability of several hESC lines maintained after prolonged in vitro culture but controversial data has been reported. Here, we prompted to compare the chromosomal stability of three hESC lines maintained in the same laboratory using identical culture conditions and passaging methods.

Results

Molecular cytogenetic analyses performed in three different hESC lines maintained in parallel in identical culture conditions revealed signifi

SUBMITTER: Catalina P 

PROVIDER: S-EPMC2567976 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature