Characterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili.

ABSTRACT:

Background

Cryptomeria japonica D. Don is one of the most commercially important conifers in Japan. However, the allergic disease caused by its pollen is a severe public health problem in Japan. Since large-scale analysis of expressed sequence tags (ESTs) in the male strobili of C. japonica should help us to clarify the overall expression of genes during the process of pollen development, we constructed a full-length enriched cDNA library that was derived from male strobili at various developmental stages.

Results

We obtained 36,011 expressed sequence tags (ESTs) from either one or both ends of 19,437 clones derived from the cDNA library of C. japonica male strobili at various developmental stages. The 19,437 cDNA clones corresponded to 10,463 transcripts. Approximately 80%

SUBMITTER: Futamura N 

PROVIDER: S-EPMC2568000 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature