Hint2, a mitochondrial apoptotic sensitizer down-regulated in hepatocellular carcinoma.

ABSTRACT:

Background & aims

Hints, histidine triad nucleotide-binding proteins, are adenosine monophosphate-lysine hydrolases of uncertain biological function. Here we report the characterization of human Hint2.

Methods

Tissue distribution was determined by real-time quantitative polymerase chain reaction and immunoblotting, cellular localization by immunocytochemistry, and transfection with green fluorescent protein constructs. Enzymatic activities for protein kinase C and adenosine phosphoramidase in the presence of Hint2 were measured. HepG2 cell lines with Hint2 overexpressed or knocked down were established. Apoptosis was assessed by immunoblotting for caspases and by flow cytometry. Tumor growth was measured in SCID mice. Expression in human tumors was investigated by microarray

SUBMITTER: Martin J 

PROVIDER: S-EPMC2569837 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature