Ontology highlight
ABSTRACT:
SUBMITTER: Shi X
PROVIDER: S-EPMC2570256 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Shi Xinghua X Basran Jaswir J Seward Harriet E HE Childs William W Bagshaw Clive R CR Boxer Steven G SG
Biochemistry 20071121 50
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluorescence anisotropy at pH 6.0 (McAnaney, T. B., Zeng, W., Doe, C. F. E., Bhanji, N., Wakelin, S., Pearson, D. S., Abbyad, P., Shi, X., Boxer, S. G., and Bagshaw, C. R. (2005) Biochemistry 44, 5510-5524). Here we report that the fluorescence anisotropy of YFP 10C depends on protein concentration in the ...[more]