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Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing.


ABSTRACT: Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the (13)C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

SUBMITTER: Oka AR 

PROVIDER: S-EPMC2570285 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing.

Oka A R AR   Phelps C D CD   McGuinness L M LM   Mumford A A   Young L Y LY   Kerkhof L J LJ  

Applied and environmental microbiology 20080829 20


Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the (13)C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field. ...[more]

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