High glucose induces toll-like receptor expression in human monocytes: mechanism of activation.

ABSTRACT:

Objective

Hyperglycemia-induced inflammation is central in diabetes complications, and monocytes are important in orchestrating these effects. Toll-like receptors (TLRs) play a key role in innate immune responses and inflammation. However, there is a paucity of data examining the expression and activity of TLRs in hyperglycemic conditions. Thus, in the present study, we examined TLR2 and TLR4 mRNA and protein expression and mechanism of their induction in monocytic cells under high-glucose conditions.

Research design and methods

High glucose (15 mmol/l) significantly induced TLR2 and TLR4 expression in THP-1 cells in a time- and dose-dependent manner (P < 0.05). High glucose increased TLR expression, myeloid differentiation factor 88, interleukin-1 receptor-associated kinase

SUBMITTER: Dasu MR 

PROVIDER: S-EPMC2570406 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature