The -786T>C promoter polymorphism of the NOS3 gene is associated with prostate cancer progression.

ABSTRACT:

Background

There is no biological or epidemiological data on the association between NOS3 promoter polymorphisms and prostate cancer. The polymorphisms in the promoter region of NOS3 gene may be responsible for variations in the plasma NO, which may promote cancer progression by providing a selective growth advantage to tumor cells by angiogenic stimulus and by direct DNA damage.

Methods

This study aimed evaluating the NOS3 promoter polymorphisms by PCR-SSCP and sequencing, associating genotypes and haplotypes with NOS3 expression levels through semi-quantitative RT-PCR, and with PCA3 mRNA detection, a specific tumor biomarker, in the peripheral blood of pre-surgical samples from 177 patients; 83 PCa and 94 BPH.

Results

Three novel SNPs were identified -764A>G, -714G>

SUBMITTER: Marangoni K 

PROVIDER: S-EPMC2571109 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature